ISSN: 0973-7510

E-ISSN: 2581-690X

Hoda Omar1, Elsayed A. Elsayed2,3, Ahmed A. Kenawy4,5, Mohamed A. Wadaan2 and Hesham A. El Enshasy5,6
1Microbiology Department, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
2Bioproducts Research Chair, Zoology Department, Faculty of Science, King Saud University, Riyadh, Saudi Arabia.
3Natural and Microbial Product Department, National Research Centre, Dokki, Cairo, Egypt.
4Faculty of Forestry, The University of British Columbia, Vancouver, BC, Canada.
5Genetic Engineering and Biotechnology Research Institute, City for Scientific Research andTechnology Applications (CSAT), New Burg Al Arab, Alexandria, Egypt.
6Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM),
81310 Skudai, Johor, Malaysia.
J Pure Appl Microbiol. 2014;8(1):121-128
© The Author(s). 2014
Received: 06/12/2013 | Accepted: 12/02/2014 | Published: 28/02/2014

This study was focused on the investigation of the kinetics of cell growth and oxytetracycline (OTC) production during the cultivation of Streptomyces rimosus in submerged culture in different scales using semi-defined cultivation medium. In shake flask cultures, the volumetric and specific antibiotic production, reachedafter 108 h, were 170 mg/L and 37.9 mg/g, respectively. Further increases in volumetric and specific OTC productions was achieved upon transferring the process to stirred tank bioreactor and studying the effect of agitation speed on the kinetics of cell growth and antibiotic production. Of different agitation speeds applied ranging between 250 and 750 rpm, the maximal cell growth of 8.15 g/L and volumetric OTC production of 410 mg/L were obtained in 500 rpm agitated culture. Further increase in agitation speed, up to 750 rpm, decreased the biomass by about 40% and the volumetric OTC production by 29%.


Streptomyces rimosus, oxytetracycline, scaling up, shear stress, submerged culture

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.