ISSN: 0973-7510

E-ISSN: 2581-690X

M. Valan Arasu1,2, S. Ilavenil2, M. Jane2, D.H. Kim3, K.D. Lee4, H.S. Park2 and K.C. Choi2
1Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies,
College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
2Grassland and forage division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam, 330-801, Republic of Korea.
3The United Graduate School of Agricultural Sciences, Tottori University, Tottori-Shi, 680-8553, Japan.
4Department of Oriental Medicine Materials, Dongsin University, Naju, 520-714, Republic of Korea.
J Pure Appl Microbiol. 2014;8(5):4017-4023
© The Author(s). 2014
Received: 04/08/2014 | Accepted: 10/09/2014 | Published: 31/10/2014

The present study aimed to investigate the effects of silage quality, microbial contents and fermentation metabolites of mixed ratio of barley and pea with the supplementation of chlorella with Lactobacillus plantarum under field conditions of livestock farmers and to monitor the responses of silage variables after forty five days. After silage preparation completed, the contents of dry matter (DM), crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), RFV (Relative feed value), crude ash (CA) and microbes such as lactic acid bacteria (LAB), yeast and fungi counts, and fermentation metabolites such as lactic acid, acetic acid and butyric acids were analyzed. Results indicated that the nutritive profiles were significantly changed with respect to the concentration of chlorella. Addition of L. plantarum further improved the silage quality. Among the microbes, LAB showed dominant and counts were recorded in barley and pea silages (6.92 and 6.99 x 107 cfu g-1) and 50 % barley with 50 % pea and 80 % barley with 20 % pea (6.869 and 4.12 x 107 cfu g-1) respectively, whereas the average numbers of yeast and fungi significantly less. The pH of the samples was ranged from 3.6-5.45. Lactic acid detected as the dominant organic acids, detected higher amount (14.59%) in 0.25% chlorella supplemented pea silage. This result confirmed that silage preparation using different crops with the supplementation of chlorella and L. plantarum inoculation is most beneficial for farmers.


L. plantarum, Chlorella, Barley, Pea, Nutritive profile, Lactic acid, Acetic acid

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.