ISSN: 0973-7510

E-ISSN: 2581-690X

Hong-Xia Yu1, Chun-Yan Wang1, Hui Chen and Ming Tang
1College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China.
J Pure Appl Microbiol. 2014;8(2):1197-1208
© The Author(s). 2014
Received: 25/10/2013 | Accepted: 06/01/2014 | Published: 31/04/2014
Abstract

In the semi-arid Loess Plateau of northwestern China, the vegetation deterioration and soil degradation are very severe, and pines are large-scale planted for reforestation. In this study, the dynamics of ectomycorrhizal (EM) fungal communities in a chronosequence of 3, 7, 12, 25 and 55 year-old Pinus tabulaeformis plantations were investigated by denatured gradient gel electrophoresis (DGGE) based on fungal internal transcribed spacers of rDNA. A total of 21 DGGE bandtypes were successfully retrieved and sequenced after molecular cloning. The results indicated that they belonged to Ascomycota and Basidiomycota, accounting for 23.81% and 76.19% of the total, respectively. The dominant genus was Russula, followed by Suillus, and then Cortinarius, Tuber and Phialophora. The DGGE profiles of EM fungal communities changed with seasons as well as plantation ages, and redundancy analysis indicated that shifts in EM fungal community composition affected by soil chemical properties was smaller among plantation ages than among seasons.

Keywords

Dynamics, Ectomycorrhizal fungi, Community, Denaturing gradient gel electrophoresis, Pinus tabulaeformis, Loess Plateau

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