Over the last decades, infections due to multi-drug resistant enterococci have been increasingly reported worldwide. This study was done so as to determine the prevalence of enterococcal infections and various species, to detect drug resistance and epidemiological pattern of the resistant isolates. The study was conducted in a tertiary care hospital in North India. Isolates of enterococci were collected from various clinical specimens and speciated using extensive phenotypic and physiological tests. Antimicrobial susceptibility testing was performed by disc diffusion and agar screening method as per CLSI guidelines. RAPD typing was done to characterize the vancomycin resistant isolates. In our study we found E. faecium as the major isolated species from the specimens from enterococcal infections. The antimicrobial susceptibility testing depicted multi-drug resistant isolates. The RAPD typing of VRE revealed heterogeneous patterns without any dominant clone. Our study showed prevalence of multi-drug resistant enterococcal isolates, with notable resistance to ampicillin, fluoroquinolones and high level aminoglycosides. Epidemiological pattern of VRE revealed acquisition of resistance determinants independently.