ISSN: 0973-7510

E-ISSN: 2581-690X

Niraj A. Ghanwate1, P.V. Thakare2 , P.R. Bhise3 and Pradnya Pimparkar1
1Department of Microbiology, Sant Gadge Baba Amravati University, Amravati, Maharashtra – 444602, India.
2Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, Maharashtra – 444602, India.
3Department of Microbiology, Dr. P D M Medical College, Amravati, Maharashtra – 444603, India.
J Pure Appl Microbiol. 2015;9(3):2611-2614
© The Author(s). 2015
Received: 06/04/2015 | Accepted: 19/08/2015 | Published: 30/09/2015

Methicillin Resistant Staphylococcus aureus (MRSA) is one of the major pathogen responsible for nosocomial and community acquired infection. High level of MRSA is present on everyday items in hospitals and can be transmitted by the hands of healthcare personnel, materials and articles used in hospitals. The present investigation aimed to determine the contamination of writing pen of doctors with MRSA in hospital environment and to assess the survival of MRSA on three kinds of new pens. Total 100 writing pens used by doctors during patient interaction were swabbed and inoculated on Blood agar.  Staphylococcus aureus were identified by standard methods. After confirmation by coagulase test, S. aureus strains were tested for their methicillin resistance by agar screen method using Muller-Hinton agar containing 6 µg oxacillin/mL and 4% NaCl. To determine the survival of MRSA on pen, three kind of new pens like metal, plastic and pen with rubber grip were smeared with 0.5 McFarland culture of MRSA, incubated and survival was determined at every  3hrs interval. Out of 100 pens analyzed, 60 were found to be contaminated with different bacteria. Gram positive bacteria were isolated from 40 pens. Staphylococci were isolated from 29 pens of which 25 were coagulase positive and 4 were coagulase negative. Out of 25 coagulase positive staphylococci, 7 were resistant to oxacillin (MRSA). MRSA survived up to 48 h on pen with rubber grip, about 30 h on plastic pen and minimum survival i.e. 21 h on pen with metal surfaces. Thus pens can carry bacteria and are fomites.


MRSA, nosocomial infections, fomites, doctor’s pen

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© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.