DNA methylation is one of the epigenetic changes that may affect cell functions, which associated with several steps of cancer progression. Gastric carcinoma is endemic in Iraq with highly association of Epstein Barr Virus infections. Epigenetic changes for gastric carcinoma diagnosis are very important to early diagnose the disease also consider as biomarker to search of the progression of cancer. So detection of methylation of early genes in Epstein barr virus associated gastric carcinoma is very useful to confirm the diagnosis of the disease. The first step is detection of the virus in the fresh and embedded tissues, then search of the methylation pattern of the early genes of the virus that expressed several important proteins that effect the life cycle if the virus also the progression of the disease using conventional polymerase chain reaction. The virus was detected in 20% of tissue samples, while the methylation state was positive in 87.5% in tissues affected with the virus. Highly percentage of the methylation in the early genes of the virus may affect the virus infection which will affect the progression of the cancer.
DNA methylation, Epigenetic change, Epstein barr virus, virus early genes, gastric carcinoma.
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