Animal and humans bear micro flora on their body surfaces and other parts exposed through various tracts to the external environment and show a great diversity. The availability of bacterial flora varies according to various environmental conditions. Present study carried out in Sriganganagar, Bikaner, Nokha and Hanumangarh, showing varying diversity of human sputum bacterial flora in various parameters. Highly male retained much bacterial diversity as compare to female. In samples, most common bacteria are Staphylcoccus aureus, Corynebacterum sp., E. coli, Klebsiella pneumoniae, Streptococcus pyogens, Pseudomonas aeruginosa, Haemophillus influenzae, Proteus vulgaris, Mycobacterium sp., Enterobacter sp., Yarsinia sp. and Nesseria sp.