ISSN: 0973-7510
E-ISSN: 2581-690X
Thermophilic microbial community in one of Kamojang hot springs, namely Kawah Hujan A, was analysis through 16S rRNA gene sequences combined with denaturing gradient gel electrophoresis (DGGE). The analysis was based on culture-independent and culture dependent strategies. Kawah Hujan A is a neutral hot spring with pH at 7.3 and low concentration of cation and anion. Microbial communities obtained from culture-independent were close to g-proteobacteria, however, detail comparison among the member of g-proteobacteria showing some sequences variation compared to the published data especially on the hypervariable and variable regions. In addition, the sequences did not belong to certain genus. Meanwhile, the 16S rDNA sequences from culture-dependent samples revealed different sequences compared to that from the culture independent sample. Phylogenetic and homological analysis of the sequences from cultivation samples showed that the sequences were close to Firmicute and Deinococcus phyla, including Geobacillus, Anoxybacillus and Thermus genera.
Thermophiles, 16S rRNA, DGGE, community analysis
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