Diversity of Free Living Nitrogen Fixing Bacteria in Sugarcane Rhizosphere of Barak Valley, Assam

ISSN: 0973-7510

E-ISSN: 2581-690X

Debamita Chakraborty , G.D. Sharma¹ and B. Deb²

¹Department of Life Science, Assam University Silchar – 788 011, India.

²Department of Botany, G.C College, Silchar – 788 004, India.

J Pure Appl Microbiol. 2012;6(3):1351-1355

Received: 21/02/2012 | Accepted: 05/04/2012 | Published: 30/09/2012

Abstract

Three different types of nitrogen fixing bacteria viz, Azotobacter vinelandii, Bacillus polymyxa and Pseudomonas fluorescens were isolated from rhizosphere of field-grown sugarcane in Barak Valley, Assam. On an average highest number of diazotrophs was recorded in rainy season, which was followed by summer season. Lowest number of diazotrophs was observed in winter season. Nitrogenase activity of Azotobacter vinelandii, Bacillus polymyxa and Pseudomonas fluorescence was 403.05, 209.15, 107.80 (nM C₂H₂/h / mg protein) respectively. Data obtained in this work will hopefully contribute to future research aimed at developing reliable and effective sugarcane inoculants.

Keywords

Diazotrophs, diversity, Sugarcane rhizosphere, nitrogenase activity

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