ISSN: 0973-7510

E-ISSN: 2581-690X

Mona Esmailzadeh1, Mahmood Saffari1, Rezvan Moniri1,
Hamid Reza Gilasi2 and Marzieh Jabbary1
1Department of Microbiology and Immunology, School of Medicine,
Kashan University of Medical Sciences, P.O. Box 8715988141, Kashan, Iran.
2Department of Biostatistics and Epidemiology, School of Health, Kashan University of Medical Sciences, Kashan- Iran. P.O. Box 8715988141, Kashan, Iran.
J. Pure Appl. Microbiol., 2016, 10 (3): 1919-1926
© The Author(s). 2016
Received: 17/03/2016 | Accepted: 20/05/2016 | Published: 30/09/2016
Abstract

The aim of this study was to determine, the rate and molecular characterization of aminoglycoside resistance genes (aac(6′)-Ie-aph(2’’)-Ia, aph(2’’)-Ib, aph(23 )-Ic, and aph(23 )-Id) among high level gentamicin resistance (HLGR) enterococcus isolates in Kashan, Iran. A total of 180 enterococcus species were tested for high level gentamicin resistance by using disk diffusion method and minimum inhibitory concentration (MIC>500 ¼g/mL) confirmatory test. High level gentamicin resistance strains were further assessed for aminoglycoside resistance genes. Antibiotic susceptibility pattern revealed that 43 isolates (23.9%) were high level gentamicin resistance (HLGR) (MIC>500¼g/ml), 24 isolates (55.8%) of HLGR isolates were resistant to Chloramphenicol, 13 isolates (30.2%) to Quinupristin-dalfopristin, 7 isolates (16.3%) to Linezolid and 9 isolates (20.9%) of HLGR isolates were multi-drug resistant. The PCR method revealed that 76.7% of high level gentamicin resistance isolates carried aac(62 )Ie-aph(23 )Ia gene; but aph(23 )Ib, aph(23 )Ic, and aph(23 )Id genes were not detected among our isolates. The aac (6′)-Ie-aph (2’’)-Ia was detected in (71.9%) and (28.1%), of Enterococcus faecalis and E. faecium, respectively. These results point to that high level aminoglycoside resistance genes are extensively disseminated among ICU isolates of enterococci.

Keywords

Enterococcus, aminoglycoside, Intensive Care Unit.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.