Differential Between Multi-Drug Resistance Pattern of Extended Spectrum β- Lactamases Producing E. coli and K. pneumoniae

ISSN: 0973-7510

E-ISSN: 2581-690X

Differential Between Multi-Drug Resistance Pattern of Extended Spectrum β- Lactamases Producing E. coli and K. pneumoniae

Sobhan Ghafourian^1,2, Sara Soheili², Elham Aboualigalehdari³, Nourkhoda Sadeghifard¹, Reza Mohebi¹, Abbas Maleki¹, Rukman Awang Hamat², Shiva Hosseini¹ and Zamberi Sekawi²

¹Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.

²Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia.

³Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

J Pure Appl Microbiol. 2014;8(Spl. Edn. 1):85-89

Received: 20/02/2014 | Accepted: 26/04/2014 | Published: 31/05/2014

Abstract

The current study aimed to determine the prevalence of Extended-Spectrum b-Lactamases (ESBLs) in Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) strains isolated from patients with Urinary Tract Infections (UTIs), to study the association between presence of ESBL enzyme and multi-drug resistance strains and finally, and to investigate the predominant ESBL gene in E. coli and K. pneumoniae. The strains were examined for the presence of ESBL as a Clinical Laboratory Standards Institute (CLSI) guideline. Among 284 clinical isolates, 52.8% (n = 150) and 47.2% (n =134) were E. coli and K. pneumoniae, respectively, and 110 strains were ESBL producer, which 68 strains were K. pneumoniae and 42 strains were E. coli. Significant difference observed between the TEM gene and ciprofloxacin resistant in E. coli (P £ 0.05) while no significant difference observed between CTX-M, SHV genes and the other multi-drug resistant E. coli. No significant difference observed between CTX-M, TEM, and SHV genes and multi-drug resistant K. pneumoniae. In conclusion, spreading of ESBL-producing strains is a concern, as it causes limitations to the antimicrobial agents for optimal treatment of patients. Prevalence of ESBLs was more observed in K. pneumoniae than E. coli. In addition, TEM

Keywords

Multi-drug Resistant, ESBLs, Urinary Tract Infection

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