ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Mohammad A. Hamad , A.M. Al-Aalim and Aamer Y.H. Alchalaby
Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
J. Pure Appl. Microbiol., 2019, 13 (4): 2273-2279 | Article Number: 5878
https://doi.org/10.22207/JPAM.13.4.41 | © The Author(s). 2019
Received: 25/09/2019 | Accepted: 02/11/2019 | Published: 19/12/2019
Abstract

Starlings have tremendous economic and environmental impacts because they can spread pathogens to livestock and poultry. These birds act as mechanical and biological carriers for different types of pathogens from and to their original habitat. The goal of this study was to ascertain the presence of Mycoplasma gallisepticum (MG) in the starlings’ lungs and confirm their diagnosis using the PCR technique. We altered the supplements that were added to Mycoplasma culture media by using calf serum instead of horse serum and sulphadimidine plus trimethoprim with nystatin instead of thallous acetate. Eighty-five starlings were bought from hunters in the spring of 2019, and their lungs were harvested and divided into two portions, one for Mycoplasma cultivation and the other for DNA extraction. Fifty-nine (69.4%) samples were positive for Mycoplasma colonies, thereby yielding accurate results using alternative supplements in the culture media. PCR revealed the presence of Mycoplasma in 78.8% lung samples, while MG was detected in only 43.3% of the positive samples, indicating the presence of other species of Mycoplasma too. The current study is the first of its kind not only in Iraq but also in the world, investigating the presence of MG in the lungs of starling birds. This study revealed that MG is significantly prevalent in starlings and also suggests that other Mycoplasma species may be present in starlings.

Keywords

Starlings, Mycoplasma gallisepticum, PCR, culturing, calf serum.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.