H. pylori has been recognized as the principle cause of peptic ulcer disease and the main risk factor for development of gastric cancer. Up to 95% of patients with duodenal ulcer, and 80% of the patients with gastric ulcer are infected by H. pylori. The diagnosis of H. pylori infection can be made by using several invasive (culture, histological stains and rapid urease tests) and non-invasive techniques (serology, urea breath test or stool antigen detection). Eradication of this organism leads to ulcer healing and markedly lowers the incidence and recurrences. This study was conducted to compare, the different diagnostic methods in the local population of Kanpur to select the most effective test for the diagnosis of H. pylori. Biopsy specimens were taken from antral mucosa of 100 patients reported to endoscopic unit with complaints of APD. The biopsy specimens were tested for H. pylori infection by RUT. Serum IgG against H. pylori (sIgGHp) was detected by ELISA. Forty six per cent cases were positive by serology test and 45% cases were positive by RUT. Serology and RUT had the best sensitivity and specificity. Non-invasive techniques like serology can be as sensitive as invasive technique like RUT. Serology and RUT can be used to confirm clinical diagnosis of duodenal erosion and ulcer.