Development and Validation of a Spectrophotometric Method for Estimation of Thyroxine Sodium in Bulk and in Tablet Dosage form

ISSN: 0973-7510

E-ISSN: 2581-690X

C. Bala Sekaran¹ , A. Prameela Rani², B.B. Pawan Kumar³, P.S.K. Bhargav³ and B.N.V.R. Kinnera³

¹Department of Biotechnology, P. B. Siddhartha College of Arts and Science, Vijayawada – 520 010, India.

²Department of Pharmaceutics, K.V.S.R. Siddhartha College of Pharmaceutical Sciences,
Vijayawada – 520 010, India.

³Department of Microbiogy, P. B. Siddhartha College of Arts and Science, Vijayawada – 520 010, India.

J Pure Appl Microbiol. 2008;2(2):551-553

Received: 06/04/2008 | Accepted: 15/08/2008 | Published: 31/10/2008

Abstract

A simple and sensitive spectrophotometric method for the determination of thyroxine sodium was developed. This method was based on condensation of the primary amino group of thyroxine sodium with acetyl acetone and formaldehyde producing a yellow coloured product, which is measured spectrophotometrically at 430nm. The colour was stable for at least 5 hours. Beer’s law was valid within a concentration range of 10 – 50 µg/mL. No interference was observed in the presence of common pharmaceutical excipients. The validity of the method was tested by analyzing thyroxine sodium in its pharmaceutical preparations. Good recoveries were obtained. This method was successfully employed for the determination of thyroxine sodium in various pharmaceutical preparations and biological samples.

Keywords

Tyroxine sodium, spectrophotometric analysis, acetyl acetone/ formaldehyde reagent, Sandell’s sensitivity

Download PDF

Article Metrics

Article View: 724

JPAM.2.2.46 (251 downloads)

Share This Article

© The Author(s) 2008. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.