ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Hanaa Jaffer Jabbar Alkabee1 , Adnan Kareem Alsalami1 and Batool Mahmod Muhamed Alansari2
1Clinical Laboratory Science Department, Pharmacy College, Kufa University, Kufa, Iraq.
2Food Science Department, Agriculture College, Basrah University, Basrah, Iraq.
J. Pure Appl. Microbiol., 2020, 14 (1): 437-446 | Article Number: 6017
https://doi.org/10.22207/JPAM.14.1.45 | © The Author(s). 2020
Received: 31/12/2019 | Accepted: 18/02/2020 | Published: 07/03/2020
Abstract

Bacterial lipases are the most important collection of biocatalysts used for a variety of biotechnological applications. In the current study the morphological, biochemical and Molecular characteristics to the extracellular lipase producing bacteria were identified. The bacterial isolates were selected as lipase producing bacteria using Rhodamine-B agar plate. the  production of Lipase from the organism were determined with varying incubation temperature (20 to 50°C) and range of incubation time (16-120) hrs. 16 bacterial isolates were identified as Staphylococcus hemolytic. One isolate “St1” was identified By comparing the observed DNA sequences of these local samples with the retrieved DNA sequences (GenBank acc. MG595372.1) as showed high lipase activity according to experimental conditions about 140  U/ml. The current study showed that enzymatic activity of crude lipase extract was maximum in 72 hrs incubation period and 37°C.

Keywords

Lipase, Staphylococcus hemolytic, Genetic Identification, Lipase extraction, Enzyme activity.

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