ISSN: 0973-7510

E-ISSN: 2581-690X

G. Vijaya Raja1 , S. Lalitha Devi2, P. Raj Kumar3 and K.R.S. Sambasivarao4
1Centre for Biotechnology, Acharya Nagarjuna University, Nagarjunanagar, India.
2Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences, Tirupati, India.
3Department of Biochemistry, N.T.R. Veterinary College, Gannavaram, India.
4Centre for Biotechnology, Acharya Nagarjuna University, Nagarjunanagar, India.
J Pure Appl Microbiol. 2009;3(1):367-370
© The Author(s). 2009
Received: 12/11/2008 | Accepted: 27/12/2008 | Published: 30/04/2009

The present work describes two new spectrophotometric methods for the determination of phenazopyridine hydrochloride (PPH) in bulk and formulations. The methods are based on the reduction of Fe3+ into Fe2+ by the drug followed by the complex formation of Fe2+ with 1,10 phenanathroline (method A) and  2,2′ bipyridyl (method B) to form  red colored chromogen having maximum absorbance at 530nm and 520nm for methods A and  B respectively. Beer’s law is obeyed in the range of 2-10 ?g/mL with molar absorptivity of 2.185 × 104 for method A and 2.305 X 104 for method B respectively. The results were favorably compared with those obtained by reference UV spectrophotometric method. The accuracy, precision, and the linearity ranges of the methods have been determined, and they have been validated by analyzing the title drug. No interference was observed from common pharmaceutical adjuvants.These two methods were successfully applied to the pharmaceutical formulations, capsules, tablets.


Sandell’s sensitivity, Phenazopyridine, Beer’s law

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© The Author(s) 2009. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.