ISSN: 0973-7510

E-ISSN: 2581-690X

N. Rahimifard1,2 , Sh. Shoeibi1,2, M. Pirali Hamedani1,2, SR. Pakzad1,2, S. Ajdari3, H. Hajimehdipour1,2, F. Bagheri4, B. Mohamadpour4 and S. Shahbazi4
1Food and Drug Laboratory Research center (FDLRC), Tehran, Iran.
2Food and Drug Control Laboratories (FDCLs), Ministry of Health (MOH), Tehran, Iran.
3Immunology Department, Pasteur Institute, Tehran, Iran.
4Microbiology Department, Pharmaceutical sciences branch, Islamic Azad University, Tehran, Iran.
J Pure Appl Microbiol. 2009;3(1):63-65
© The Author(s). 2009
Received: 12/06/2008 | Accepted: 24/08/2008| Published: 30/04/2009
Abstract

Mycoplasma is a genus of bacteria that lack a cell wall. Because they lack a cell wall, they are unaffected by some antibiotics such as penicillin or other beta-lactam antibiotics that target cell wall synthesis.Mycoplasma is a major contaminant of cell lines and is considered as a serious economic and biologic problem in the basic research, biotechnology products such as vaccines and laboratories diagnosis.12 types of OPV vaccines in 8 series were randomly collected from the post marketing aria. Standard culture method for Mycoplasma detection in biological substances, based on BP (A190), EP (2.6.7), VICH GL34 for detection of Mycoplasma contamination was performed. None of 96 samples were positive by culture methods while the results of positive and negative controls and performance tests for culture media were acceptable.

Keywords

Mycoplasma, Vaccines, Standard culture method

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© The Author(s) 2009. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.