Mycoplasma is a genus of bacteria that lack a cell wall. Because they lack a cell wall, they are unaffected by some antibiotics such as penicillin or other beta-lactam antibiotics that target cell wall synthesis.Mycoplasma is a major contaminant of cell lines and is considered as a serious economic and biologic problem in the basic research, biotechnology products such as vaccines and laboratories diagnosis.12 types of OPV vaccines in 8 series were randomly collected from the post marketing aria. Standard culture method for Mycoplasma detection in biological substances, based on BP (A190), EP (2.6.7), VICH GL34 for detection of Mycoplasma contamination was performed. None of 96 samples were positive by culture methods while the results of positive and negative controls and performance tests for culture media were acceptable.
Mycoplasma, Vaccines, Standard culture method
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