A total of 680 serum samples collected from both cattle (No. 544) and buffaloes (No. 136) in an organized farm were initially screened in a qualitative MAT using 12 strains of L interrogans and subsequently tested in quantitative MAT. Of which 26 sera (cattle and buffaloes inclusive) in various antibody titres were subjected to following treatments (inactivation at 560C for 30 minutes, inactivation at 65°C for 1h and treatment with 2 ME (Adler, 1965). The titres obtained after treatments and before treatments were compared. Of the 26 samples, 20 (76.92%) sera samples showed reduction in MAT titre after inactivation at 65°C for 1h and 23 (88.46%) sera samples after 2 ME treatment. The reduction in titres of 20 sera due to inactivation at the 65 °C and of 23 sera due to 2 ME treatment indicated the presence of IgM in the sera samples which suggests the recent (acute) infection. Hence this study would be useful to differentiate the acute infection of leptospirosis from the chronic case of leptospirosis.
Bovines, Leptospira, IgM antibody
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