ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Arveen Sokhi1, Priti Agarwal1 , Megha Maheshwari2 and Anita Chakravarti1
1Department of Microbiology, FMHS, SGT University, Budhera, Gurugram- Badli road, Gurugram- 122505, Haryana, India.
2Department of Microbiology, Dr. Baba Saheb Ambedkar Medical College and Hospital, Sector-6, Rohini, New Delhi-110085, India.
J. Pure Appl. Microbiol., 2020, 14 (1): 397-401 | Article Number: 5995
https://doi.org/10.22207/JPAM.14.1.41 | © The Author(s). 2020
Received: 04/12/2019 | Accepted: 29/03/2020 | Published: 31/03/2020
Abstract

Carbapenem resistance is increasing and emerging as a public health threat. The Indian subcontinent serves as a reservoir for all 3 types of carbapenemases: KPC, OXA-181 and NDM. The present study was done to determine the antibiotic resistance pattern of Gram negative bacilli (GNB) belonging to family Enterobacteriaceae and molecular detection of blaKPC gene among the Carbapenem resistant Enterobacteriaceae (CRE). Antibiotic sensitivity pattern of 301 gram negative isolates, members of Enterobacteriaceae (E. coli, K. pneumoniae, K. oxytoca, Proteus species, Citrobacter species, Enterobacter species and Serratia species) was determined and those resistant to carbapenem (ertapenem) were further processed in the study. Modified Hodge Test (MHT) was performed to phenotypically confirm the presence of carbapenemases (blaKPC) and Polymerase Chain Reaction (PCR) was done to identify blaKPC gene. Of the total 301 isolates, 45 were resistant to ertapenem. Out of these 45 isolates 32 were MHT positive and three of these 32 MHT positive strains harboured blaKPC gene.

Keywords

blaKPC, Enterobacteriaceae, Gram negative bacilli, Carbapenem resistance, Modified hodge test.

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