Gastric cancer is forth leading cause of death world wide. Among various causative agents one of them is H.pylori and is one of the world’s most common bacterial infections, its natural habitat is the gastric mucosa PCR is a rapid, sensitive and accurate method for the specific detection of Helicobacter pylori in a variety of specimens. We compared the sensitivity of PCR that amplifies the highly conserved regions of ureA and ureC genes of H.pylori to detect the presence of H.pylori in the gastric biopsy specimens. A total of 50 gastric astral biopsy specimens were collected from dyspeptic patients of different age group. Presence of H.pylori in gastric mucosa was investigated by PCR. Twenty-five out of 50 samples were positive for ureA gene and 10 out 50 samples were positive for ureC gene with the positive predictive value 50% and 20% respectively. The prevalence rate was recorded 72%. amongst these males and females ratio were 77.2:67.8% by PCR method. The incidence of infection is high in 10-30 years of age as compare to old age group. It is concluded from the study that the ureA gene is more sensitive for the detection of H. pylori than ureC gene. We therefore recommend the use of ureA gene based PCR for clinical diagnosis.
H. pylori, PCR, Biopsy and Prevalence
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