ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Aamer Yehya Hamid Al-Chalaby
Department of Microbiology, College of Veterinary Medicine, University of Mosul, Iraq.
J. Pure Appl. Microbiol., 2020, 14 (1): 383-388 | Article Number: 5890
https://doi.org/10.22207/JPAM.14.1.39 | © The Author(s). 2020
Received: 02/10/2019 | Accepted: 03/01/2020 | Published: 07/03/2020
Abstract

The aim of the present study is the detection of red beef meat (local and imported) contaminated by E. coli. The microbial load in local and imported beef meat was detected by counting the E. coli total number according to the universal standards. Sixty samples of local and imported beef meat from Indian origin were collected from city meat retailers and butchers that divided into 6 localities (quarters) including AL-Hadbaa, AL-Baladiat, AL-Faisalia, AL-Majmoaa, AL-Jazair and AL-Wahdaa in Mosul city. Ten samples were collected from each locality (five local samples, five imported ones). All samples were subjected for morphological, biochemical (including API20E) and confirmed by molecular identification (PCR ). Results were revealed that imported beef samples were more contaminated with E. coli bacteria than local samples since they ranged from 61 x 102 – 22 x 103 CFU /g and 13 x 102 to 65 x 102 CFU / g respectively.

Keywords

PCR, E. coli, Bacterial count, Microbial contamination, Beef meat.

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