ISSN: 0973-7510

E-ISSN: 2581-690X

Joy L. Kataria1 , T.K. Dutta2, P. Roychoudhury3 and J.G. Tiwari4
1College of Veterinary Sciences & Animal Husbandry, Central Agricultural University, Aizawl, Mizoram- 796014, India.
J Pure Appl Microbiol. 2014;8(3):2383-2388
© The Author(s). 2014
Received: 17/11/2013 | Accepted: 24/01/2014 | Published: 31/06/2014
Abstract

A total of 100 fecal samples from  less than 3 month old piglets with (n=60) or without (n=40) diarrhoea were collected  and processed for isolation of Escherichia coli and screened for the presence of E. coli. A total of 254 E. coli isolates  obtained were subjected to multiplex polymerase chain reaction (m-PCR) based screening of stx1, stx2, eaeA and hlyA genes. Out of 254 isolates, presence of at least one virulence gene were detected in 51 (20.08%) isolates of which 30 (11.81%) and 21 (8.26%) isolates were  identified as shiga toxin producing (STEC) and enteropathogenic E. coli (EPEC), respectively. The antimicrobial sensitivity pattern of the STEC and EPEC isolates obtained in the present study revealed prevalence of high level of resistance against bacitracin, methicillin, novobiocin, lomefloxacin, kanamycin, amikacin, ciprofloxacin and spectinomycin.

Keywords

EPEC, HUS, STEC, TTP

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