ISSN: 0973-7510

E-ISSN: 2581-690X

S.K. Shukla1 , A Chauhan1, S. Shukla2, M. Panigrahi1, B. Bhushan1, S. Maurya1, Sarvjeet1, R. Maurya1, Vinay K. Saxena1, S. Kumar1, C. Prakash3 and R.V. Singh1
1Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly- 243122, India.
2Department of Biosciences, Integral University, Lucknow, U.P., India.
3CADRAD, Indian Veterinary Research Institute, Izatnagar, Bareilly, India.
J. Pure Appl. Microbiol., 2016, 10 (2): 1579-1584
© The Author(s). 2016
Received: 25/11/2015 | Accepted: 18/02/2016 | Published: 30/06/2016

Bovine tuberculosis (bTB) in cattle is a serious zoonotic disease of worldwide distribution. Bovine tuberculosis is increasingly prevalent among farmed cattle in India, exerting a heavy economic burden on the farming industry and Government. This study was aimed to determine the prevalence of bovine tuberculosis in Indian cattle (Bos indicus), based on Post-mortem, Ziehl-Neelsen, Mycobacterial culture and ELISA. In post-mortem were found eight positive samples, twelve negative samples and three doubtful samples. With the help of Ziehl-Neelsen technique fifteen cattle sample were screened, seven positive samples, six negative samples and two doubtful samples. Thirty two cattle sample were identified based on mycobacterial culture, eleven positive samples, fourteen negative samples and seven doubtful samples. Sixteen positive samples, thirteen negative samples and thirty one doubtful samples were detected in ELISA test with M. bovis standard positive and control serum sample. The study found a high infection rate of bTB among cattle and majority of the lesions were found on lungs. We conclude that different laboratory testing requires the combination of a set of available diagnostic tools for better sensitivity and specificity of results.


Post-mortem, Z-N Technique, Mycobacterial culture, ELISA.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.