In order to define the define the minimal region of PRKT17 promoter previously isolated necessary for activity, deletions were carried out from 5’ end of PRKT17 promoter based on the basic characteristics of promoters. Taking this as a reference A3 was designed. The E.coli clone pGBK111 containing A3 region could derive the resistance of E coli. to level of 250µg per ml of chloramphenicol.
Deletion, Promoter, Region
Share This Article
© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.