Biodegradation of hydrocarbon-contaminated soils has been established as an efficient, economic, versatile and environmentally sound treatment. A total of nineteen morphologically different bacterial cultures were isolated. The growth of isolated cultures was studied in liquid M9 medium containing 0.5 % 2T engine oil. On the basis of increased OD in M9 medium, bacterial strains GD₂ was selected for further study. On the basis of morphological, physiological and biochemical properties strain GD₂ was tentatively identified as Acinetobacter sp.  It was found that the GD₂ strain degraded the following hydrocarbons (Trans, trans-1-6-dimethylspiro [4, 5] decane, Cis trans-1-6-dimethyl spiro [4, 5] decane, Pyrrolo [1, 2-a] pyrazine-1, 4-dione, hexahydro-3-(2-methylpropyl), Octateiacontyl pentafluoropropionate, Octacosyltri fluoroacetate, Triacontylacetate, Tetra triacontyl pentafluropropionate, Tetra triacontyl trifluoroacetate and Tetradecane, 1 bromo) present in 2T engine oil. Capability to degrade hydrocarbons makes it potential organism for consideration in the bioremediation of by 2T engine oil contaminated sites.