ISSN: 0973-7510

E-ISSN: 2581-690X

Yasemin Gulecal-Pektas
Istanbul University, Institute of Science and Engineering, Istanbul, Turkey.
J. Pure Appl. Microbiol., 2016, 10 (2): 1607-1612
© The Author(s). 2016
Received: 10/03/2016 | Accepted: 20/04/2016 | Published: 30/06/2016
Abstract

The microbial diversity was investigated in hot spring of Cekirge, Kemalpasa, Orhaneli and Oylat (Temperature: 48-68°C, pH 7.4-7.8) in Bursa, 2014 using an integrated approach that included enrichment culturing and 16S rRNA gene pyrosequencing. Enrichment cultures for both aerobic and anaerobic microorganisms were successfully obtained at temperatures ranging from 50°C to 70°C. An average of 1,296- 2,365 operational taxonomic units per sample observed. The bacterial community was composed of sequence reads moderately related (nucleotide identity > 90%) to 36 phyla and 6 candidate phyla, with Proteobacteria, Chloroflexi, Bacteroidetes and Chlorobi being dominant. This study results provide evidence of the necessity to combine both culture-dependent and culture-independent methods for better understanding of microbial communities in hot springs.

Keywords

Enrichment culturing, Pyrosequencing, Microbial community, Hot spring, Bursa.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.