The microbial diversity was investigated in hot spring of Cekirge, Kemalpasa, Orhaneli and Oylat (Temperature: 48-68°C, pH 7.4-7.8) in Bursa, 2014 using an integrated approach that included enrichment culturing and 16S rRNA gene pyrosequencing. Enrichment cultures for both aerobic and anaerobic microorganisms were successfully obtained at temperatures ranging from 50°C to 70°C. An average of 1,296- 2,365 operational taxonomic units per sample observed. The bacterial community was composed of sequence reads moderately related (nucleotide identity > 90%) to 36 phyla and 6 candidate phyla, with Proteobacteria, Chloroflexi, Bacteroidetes and Chlorobi being dominant. This study results provide evidence of the necessity to combine both culture-dependent and culture-independent methods for better understanding of microbial communities in hot springs.