ISSN: 0973-7510

E-ISSN: 2581-690X

Santosh Kumar1 , Amarendra Kumar1, Gireesh Chand1, S.K. Biswas2 and Rakesh Kumar3
1Department of Plant Pathology, Bihar Agricultural University, Sabour – 813 210 (Bhagalpur), Bihar.
2Department of Plant Pathology, C.S. Azad University of Agriculture & Technology, Kanpur – 208 002, Uttar Pradesh, India.
3Department of Soil Science and Agricultural Chemistry, Bihar Agricultural University, Sabour – 813 210 (Bhagalpur), Bihar, India.
J Pure Appl Microbiol. 2015;9(2):1063-1068
© The Author(s). 2015
Received: 06/04/2015 | Accepted: 19/05/2015 | Published: 30/06/2015
Abstract

Investigations were conducted to find the suitable media, effect of temperature and pH on growth of Fusarium oxysporum f. sp. ciceri. Chickpea meal medium was found to be best medium for growth and sporulation of Fusarium oxysporum f. sp. ciceri followed by Potato Dextrose Agar and Corn meal medium. Growth was significantly influenced at different temperatures and pH and maximum radial growth of 81 mm was recorded at a temperature of 28 °C followed by 78 mm at 25 °C and over a pH range of 6.5. Three bioagents viz., T. viride, T. hazanium and P. fluorescens and two fungicides thiram (0.05%) and metiram (0.1%) were used in combination with T. viride as seed treatment in pots against Fusarium oxysporum f. sp. ciceri, the incitant of wilt of chickpea. Significantly minimum (14.48 %) incidence of wilt was observed in combined seed treatment of metiram (0.1%) + T. viride and 68.55 per cent disease control which was at statistically at par with Thiram (.05%) + T. viride alone which showed 16.45 per cent incidence.

Keywords

Chickpea, Fusarium oxysporum f sp. ciceri, culture media, temperature, pH, management

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