Ten isolates of Fusarium oxysporum f.sp. lycopersici collected from different tomato growing areas of south Gujarat and designated as SGFOL-1 to SGFOL-10. Studies were made on cultural and morphological variation like mycelial colour, mycelial growth, dry mycelium weight, sporulation, conidial size and formation of chlamydospores. The isolates produced moderate, profuse fluffy, thin flat to slight fluffy and submerged growth with pigmentation range of white, yellow, light pink, dark pink, orange and purple with orange pigmentation. Sporulation varied from 2.77 × 10⁶ spores/ml to 21.68 × 10⁶ spores/ml. The macro conidia ranged from 15.46-21.8 × 4.91-5.45 µm to 21.42-44.28 × 7.35-9.14 µm. The micro conidia varied from 3.57-14.28 × 2.68-4.46 µm to 7.14-14.28 × 3.57-5.35 µm. Width of mycelia and chlamydospore dimension also varied in all isolates. The maximum dry mycelium weight was observed in SGFOL-6 (193.33 mg) whereas minimum dry mycelium weight was observed in isolate SGFOL- 9 (120.67 mg). Dry mycelium weight varied from 55.33 mm to 88.33 mm.