In this research work, the cultural and microscopic characteristics of the yeast, Trichosporon mycotoxinivorans were studied using different types of media, e.g., corn meal agar, mycology broth, sabouraud dextrose agar, Czapek dox, yeast mold agar and examined by light microscope. Biochemical characteristics were studied by analytical profile index (API 20 aux strip).  Effect of the yeast on the period till fertilization and the duration of pregnancy, number of births, mortality and birth weight rate of pups were determined. The results indicated that the yeast can grow fast in many type of media utilizing simple source like glucose and peptone. The yeast has globose, ovoidal, ellipsoidal, elongate single or chain cell, and produce arthrospores. It has giant septate cells and produce true hyphae. The yeast has ability to metabolize  D-glucose, calcium-2-keto-gluconate, L-arabinose, D-xylose, D-galactose, inositol, N-acetyl-glucosamine, D-celiobiose, D-maltose, D-sucrose, D-lactose, D-trehalose, D-melezitose, D-rafinose, methyl-a-D-glucopyranoside and glycerol. However it failed to  metabolize  adonitol, xylitol and D-sorbitol compounds. It increased the fertility rate in mice female and birth weight of pups, and reduced the mortality rate of pups. The results suggest that this yeast could be used as a probiotic in animal feeding to enhance reproductive characters and survivability of pups.