ISSN: 0973-7510

E-ISSN: 2581-690X

Manal M. AlKhulaifi, Nagwa M. Aref , Ali A. AlSalamah and Mohammed. S. Al Shammary
1Department of Botany and Microbiology, College of Science, King Saud University,
P.O.Box 22452.Riyadh 11495, Kingdom of Saudi Arabia.
J Pure Appl Microbiol. 2014;8(2):1265-1274
© The Author(s). 2014
Received: 26/08/2013 | Accepted: 02/11/2013 | Published: 31/04/2014
Abstract

Staphylococcus aureus is one of the major causes of community and hospital acquired infections. As well as bacteriophage considered as a major risk factor acquires S. aureus new virulence genetic elements for it.  A total number of 119 S. aureus isolates obtained from Riyadh Military Hospital. And were studied for phage typing and the incidence of toxin genes by PCR. Methicillin Resistant S. aureus isolates (MRSA) indicated high special prevalence of phage group II with a highly increase for phage type Ø3A compared to MSSA. Phage group II on Methicillin Sensitive S. aureus isolates (MSSA) considered an epidemiologic marker with frequent strong reaction compared to group III and phage group I. Phage type Ø75 may play an important role in a combination with Ø80 or/ Ø81 by having PVL toxin to be CMRSA lineages. 68% of S. aureus isolates had toxins. The most prevalent toxins were SEO, in 50.8% in MSSA & 25% in MRSA isolates. SEI was detected in 40.3% in MSSA & 29.1% in MRSA isolates. Also, SEA was 28% in MSSA & 33.3% in MRSA isolates. Phenotypic and genotypic variations between MSSA isolates seemed to be horizontally, while in MRSA isolates were vertically. It was obvious that five toxins together were located in more than one isolates in MSSA.

Keywords

Staphylococcus aureus, Phage types, Toxins, epidemiologic marker, MSSA, MRSA

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.