Innovative approaches will be required to improve the methods for controlling and destruction of certain plant pathogens. Synthesis of nanoparticles with interesting physico-chemical properties using efficient as well as eco-friendly technology is one of the main objectives of nanotechnology. In the current study, Streptomyces bikiniensis  was isolated from soil, Riyadh, Saudi Arabia, and identified by 16S rRNA. This study showed that the shape of silver nanoparticles (AgNPs) was predominantly monodispersed and spherical shapes in the size range of 5–35 nm upon addition of 1 mM silver nitrate. The AgNPs were characterized by determining Fourier transform infrared spectroscopy (FTIR), Energy Dispersive Spectroscopy (EDS) and transmission electron microscopy (TEM). Furthermore, the biosynthesized AgNPs at 5µl significantly inhibited the growth of one of the important Gram-negative bacterium Pseudomonas syringae. Thus, bioconversion of silver nanoparticles by Streptomyces bikiniensis could be employed as a potential nanoparticle biopesticides to control the plant pathogen, Pseudomonas syringae.