ISSN: 0973-7510

E-ISSN: 2581-690X

Lei Yang, Xiangguo Wang, Lanjie lei, Guangdong Zhang, Qin Xu, Jianpo Zhang, Chunnan Zhang, Jiao Yu, Pengfei Lin, Yaping Jin, Aihua Wang
1Key Laboratory of Animal Biotechnology, Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi – 712 100, P.R. China.
J Pure Appl Microbiol. 2014;8(1):55-61
© The Author(s). 2014
Received: 06/07/2013 | Accepted: 20/09/2013 | Published: 28/02/2014

To evaluate in vitro the mechanism of function of Brucella major outer membrane proteins and the interaction with cells. The Brucella outer membrane proteins Omp10, Omp16, Omp19, Omp25, Omp28, Omp31, Omp2a genes were cloned to pMD18-T simple carrier;  Liked them to the carrier pefgp – n1 for eukaryotic expression; Examined the carrier on HEK 293 cell and detected the transfection efficiency. Cloned Brucella main outer membrane proteins and constructed their eukaryotic expression vectors, further examined the transfection efficiency on HEK 293 cells. Built carriers have happened expression in HEK 293 cells. This study provided the necessary materials for later research on relationships between cells and Brucella main outer membrane proteins.


B. Melitensis S19, outer membrane proteins, Carriers construction, Eukaryotic expression

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.