ISSN: 0973-7510

E-ISSN: 3083-3087

D. G. Panpatte1 , , H. N. Shelat2 and Y. K. Jhala3
*1Ph. D. Scholar, 2Associate Research Scientist, 3Assistant Professor Department of Agricultural Microbiology, Anand Agricultural University, Anand-388 110, India.
J. Pure Appl. Microbiol., 2015, 9 (4): 3083-3087
© The Author(s). 2015
Received: 13/06/2015 | Accepted: 19/09/2015 | Published: 31/12/2015
Abstract

Biocontrol agents and phyto-extracts (botanicals) have a great promise as preventive measure against phyto-pathogens and till date negligible research attention has been to evaluate compatibility of these two bicontrol agents. In present investigation, compatibility of phyto-extracts viz. neem extract, besharam extract and mustard cake extract with native biocontrol agents (Providencia vermicola AAU PR1, Pseudomonas putida AAU PR2 and Pseudomonas fluorescens AAU PR3) was checked. All the cultures were found to be compatible with phyto-extracts of neem, besharam and mustard cake on solid agar medium showing no zone of inhibition. The isolates were further confirmed to be compatible with phyto-extract in liquid assay wherein there was no reduction in optical density of cultures was recorded. Moreover, neem, besharam and mustard cake extract up to 10 % concentration showed to increase the growth of all the three tested microbial strains. The results of the present investigation reveal the scope of simultaneous application of biocontrol bacteria and phyto-extract to get benefit of wider range of biocontrol naturally.

Keywords

extract, botanicals, biocontrol,

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© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.