ISSN: 0973-7510

E-ISSN: 2581-690X

Said Maham1, Fariba Shirvani1 , Mana Hadipour Jahromi1, Fatemeh Fallah1, Seid Sajad Razavi2, Ali Nikfarjam3 and Mohammad Rahbar4,5
1Pediatric Infections Research Centre, Mofid Children Hospital, Shaheed Beheshti University of Medical Sciences and Health Services.
2Department of Pediatric Mofid Children Hospital, Shaheed Beheshti University of Medical Sciences and Health Services.
3Ministry of Health and Medical Education Tehran, Iran.
4Department of Microbiology, Iranian Health Reference Laboratory, Ministry of Health and 5-Medical Education, Tehran, Iran.
5Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran, Iran.
J Pure Appl Microbiol. 2012;6(4):2039-2044
© The Author(s). 2012
Received: 12/04/2012 | Accepted: 30/05/2012 | Published: 31/12/2012

Bloodstream infectious diseases are prevalent in children and adults. Rapid and accurate diagnosis of bloodstream infections primary based on conventional culture results, which saves time and prevents empiric treatment. The aim of this study was to compare the results of BACTEC 9120 and conventional culture systems, on blood specimens collected from three university affiliated hospitals in Tehran, Iran. BACTEC 9120  and conventional methods were used for isolation of microorganism from specimens including  blood and other body fluid samples collected from patients hospitalized in the selected hospitals during the study period, from April to June 2009. Time for positive and negative results and hospital charge were estimated for the two culture methods. In total 747 specimens were possessed by BACTEC 9120 systems and 787 by conventional method. Patients aged between 3 days and 8  years old, (mean 11.4±21.9 years); 52% of patients were male and 48% female; Out of 747 specimens were possessed by BACTEC 9120  system, 26% (196/747) and from 787 specimens cultured by conventional method 5% (49/787) were positive (p<0.05). Hospital stay was 13.8 ± 12.9 days in BACTEC 9120 and 17.9 ± 14.9 days in Conventional method,(p<0.05) respectively. Time for positivity by BACTEC  system was 3.8 ± 1.1 Days  and 5.9 ± 2.5 days in conventional method.(p<0.05), treatment  response  showed 1.8 day earlier result in  patients that their specimens were processed by BACTEC 9120 system. Death rate in BACTEC 9120 method was 6% and 11% in Conventional method. (p>0.05).In conclusion implementation of BACTEC system for microbiologic detection of pathogens decreases the admission time and early diagnosis and treatment results are cost effective for patient management and prevention of antibiotic resistance.


Blood culture system, Sterile body fluids

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© The Author(s) 2012. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.