Indian white shrimp waste extracted by biosynthesis methods involving demineralization, deproteinization and deacetylation. In this study chitosan with high functionality was produced using mild conditions (temperature (60, 80 and 100 °C), concentration of alkaline (30, 40 and 50 %) and time (90, 195, 300 mins) of reaction in chemical method and power of microwave (300, 600 and 900 W), concentration of alkaline (30, 40 and 50 %) and irradiation time (20, 100, 180 S) in microwave method). Results showed that significant differences (P<0.05) were observed in decreasing of the viscosity and molecular weight of the chitosan samples with increasing concentration of alkali solution (30 to 50 %). DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activities of chitosan samples (15.26 to 17.77%) were observed and samples had low antioxidant activity compared with BHT (Butylated hydroxyltoluene). The antibacterial activities were examined against bacteria (Escherichia coli, Salmonella typhimurium and Staphylococcus aureus). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of chitosan showed the inhibition of the growth of various bacteria tested although it depends on the molecular weight of samples and the species of bacteria.