ISSN: 0973-7510

E-ISSN: 2581-690X

Saroj Golia, Vivek Hittinahalli, Mallika Reddy K. and Sujatha Karjigi
Dr. B.R. Ambedkar Medical College, K.G. Halli, Bangalore – 560 045, India.
J Pure Appl Microbiol. 2012;6(3):1259-1263
© The Author(s). 2012
Received: 22/07/2012 | Accepted: 25/09/2012 | Published: 30/09/2012
Abstract

Candida albicans and Candida non albicans are emerging important causative agents of fungal infections. The study was carried out to identify the  various species of  Candida using Chrom agar and cornmeal agar and compare their susceptibility pattern to fluconazole, voriconazole and flucytosine by microbroth dilution methods. Positive samples for candidiasis where collected from 122 patients at Dr BR AMC from may 2011 to July 2012, processed by routine methods, chrom agar media and corn meal agar was used to speciate and antifungal susceptibility done by microbroth dilution method. C.albicans44, C.tropicalis32,C.parapsilosis24,C. glabrata16 and C. krusei6 were  isolated. MIC values of fluconazole,voriconazole and flucytosine to C.albicans, C.tropicalis, C.parapsilosis, C.glabrata, C.krusei  were determined. There is increasing incidence of C.non albicans species infections. There also has been increase in the MIC values of fluconazole for non albicans candida., MIC values of voriconozole were not found to be increased and were in the range of 0.007-0.125ug/ml. Hence remains the drug of choice among all the azoles.  MIC values of flucytosine were in the range of 0.002-0.75ug/ml except for C.  krusei  where it is >32ug/ml. This  suggests that flucytosine also remains a good drug for treatment of candidiasis however this drug is more toxic.

Keywords

Candida,chromagar, Cornmeal agar, Flucanazole,Voriconazole, Flucytosine

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