ISSN: 0973-7510

E-ISSN: 2581-690X

R. Sujatha , Kavita Nimboor, Saroj Golia and D. Arunagiri
Department of Microbiology, Dr. B.R. Ambedkar Medical College, Kadugondanahalli, Bangalore, India.
J Pure Appl Microbiol. 2012;6(3):1443-1446
© The Author(s). 2012
Received: 29/02/2012 | Accepted: 03/04/2012 | Published: 30/09/2012
Abstract

Cefoxitin is increasingly recommended for the detection of Methicillin resistance in S.aureus, when using diffusion testing. The aim of the study was to evaluate  and compare the efficacy of Cefoxitin disc diffusion test  with  Oxacillin Screen Agar, and Oxacillin disc  diffusion  test. 100 strains of S.aureus isolated from clinical samples were used in the study.Routine antibiotic susceptibility testing were performed including Oxacillin disc(1µg), Oxacillin Screening Agar with  4% NaCl and 6µg/ml of Oxacillin were inoculated and interpreted as per standard guidelines. Cefoxitin disc diffusion was performed using 30µg disc & zone sizes were measured. Out of 100 isolates, 44 were found to be Methicillin resistant by Oxacillin diffusion test, 50 were resistant by Oxacillin screen agar method and 70 were resistant with Cefoxitin disc diffusion. Results of Cefoxitin disc diffusion  test is better than other phenotypic methods and can be used  for routine susceptibility testing of MRSA, in resource constraint setups that cannot  afford  PCR testing for mecA as a confirmatory test.

Keywords

MRSA, Cefoxitin, Oxacillin, Tertiary Care Centre

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