Aspergillus niger and Saccharomyces carlsbergensis were grown in submerged fermentation systems with sucrose concentration at 100 g/ liter and varying concentration of molasses in Basic Mineral Medium ( BMM) for production and characterization of invertase by Aspergillus niger and Saccharomyces carlsbergensis. As compare with Aspergillus niger the Saccharomyces carlsbergensis produce exceptionally large amount of extra cellular invertase in sucrose and varying conc. of molasses. The optimum temperature of inveratse for Aspergillus niger is 40° C and to that of Saccharomyces carlsbergensis is 50 ° C. The optimum pH of invertase for Aspergillus niger was found to be 4.4 and to that of for Saccharomyces carlsbergensis is 4.8 . The activity of this enzyme under these conditions is 600 µg of reducing sugar /1 min/1 ml of enzymes for Aspergillus niger and to that of Saccharomyces carlsbergensis is 5040µg of reducing sugar/1 min/1 ml of enzyme. For these two i.e Aspergillus niger and Saccharomyces carlsbergensis the specific activity was found to be 2.25 and 20.16 respectively. Immobilization was also done for getting immobilized cells activity. And finally gel electrophoresis is done to get separate protein bands.
Enzymes, Aspergillus niger, Saccharomyces carlsbergensi, Fermentation technique
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