ISSN: 0973-7510

E-ISSN: 2581-690X

Mohammed Inuwa Ja’afaru and Abiodun Anthony Onilude
Microbial Physiology and Biotechnology Research Group, Department of Botany and Microbiology, University of Ibadan, Nigeria.
J Pure Appl Microbiol. 2010;4(2):497-505
© The Author(s). 2010
Received: 12/08/2009 | Accepted: 19/10/2009 | Published: 31/10/2010
Abstract

This paper reports the enzymatic hydrolysis of alkali-treated agri-waste with xylanase from Trichoderma viride Fd18 and Aspergillus ustus Fd12 isolated from saw dust. Alkali-treated wheat bran had the highest hydrolysis rate of 35% (A. ustus Fd12 xylanase) and 48.6% (T. viride Fd18 xylanase). Saw dust from different plants have a low rate of hydrolysis. The optimal pH and temperature for hydrolysis of wheat bran by xylanase from the 2 fungi were pH 4.0 – 4.5 and 35oC. A wheat bran concentration of 6% and incubation time of 24hr increased rate of hydrolysis. The enzyme can be used to hydrolyze xylan in agricultural residues for commercial applications.

Keywords

Wheat bran, Trichoderma viride, Aspergillus ustus, hydrolysis, xylan, xylanase

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