Plant tissue culture offers a new way to solve this problem to recover the loss of rare plants and also for production of bioactive components from cultured cells and tissues. In present study Gymnema sylvestre leaves and callus was compared for their antimicrobial potentials to assess the feasibility of cell and tissue culture to replace the plants use from natural resources. Callus was induced by using young as explants on MS medium supplemented with 2,4-D and BA. Various solvents s Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis and Streptococcus mutans were tested for antimicrobial activity. Methanolic extract of leaves showed antimicrobial activity against Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis in agar well diffusion assay, but no activity against Staphylococcus hominis and Streptococcus mutans.
Callus, pathogenic bacteria, leaves extract
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