ISSN: 0973-7510

E-ISSN: 2581-690X

B. Sri Ramya, P. Janaki Ramayya and S.A. Mastan
Post Graduate Department of Biotechnology, P.G. Courses & Research Centre, D.N.R. College, Bhimavaram, West Godavari Dt – 534 202, India.
J Pure Appl Microbiol. 2009;3(2):573-581
© The Author(s). 2009
Received: 18/02/2009 | Accepted: 21/04/2009| Published: 31/10/2009
Abstract

In the present investigation attempts have been made to clone the NiCoT gene of N.crassa. For this purpose, the RNA was isolated from N. crassa and it was converted into cDNA. The nct gene was amplified using the gene specific primers. The genomic and protein sequences of nct genes have traced out using the FCGS web page and the sequences have been reported. It has also been observed that the rate of transportation in N.crassa is enhanced by the action of NiCoT gene.  The transmembrane domains of the nct gene were identified using the TMpred software.

Keywords

Cloning, Nickel, Cobalt, NiCoT transporter gene, Neurospora crassa

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