ISSN: 0973-7510
E-ISSN: 2581-690X
In the present investigation attempts have been made to clone the NiCoT gene of N.crassa. For this purpose, the RNA was isolated from N. crassa and it was converted into cDNA. The nct gene was amplified using the gene specific primers. The genomic and protein sequences of nct genes have traced out using the FCGS web page and the sequences have been reported. It has also been observed that the rate of transportation in N.crassa is enhanced by the action of NiCoT gene. The transmembrane domains of the nct gene were identified using the TMpred software.
Cloning, Nickel, Cobalt, NiCoT transporter gene, Neurospora crassa
© The Author(s) 2009. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.