Cloning and Expression of Dissimilatory Sulfite Reductase (dsrAB) of Desulfotomaculum geothermicum Isolated from Indian Petroleum Refinery Cooling Towers

ISSN: 0973-7510

E-ISSN: 2581-690X

Cloning and Expression of Dissimilatory Sulfite Reductase (dsrAB) of Desulfotomaculum geothermicum Isolated from Indian Petroleum Refinery Cooling Towers

B. Anandkumar¹ , E.S. Challaraj Emmanuel² and S. Maruthamuthu³

¹Department of Biotechnology, Sourashtra College, Madurai – 625 004, India.

²Department of Microbiology, Sourashtra College, Madurai – 625 004, India.

³Corrosion Protection Division, Central Electro-Chemical Research Institute, Karaikudi – 630 001, India.

J Pure Appl Microbiol. 2008;2(2):381-386

Received: 13/07/2008 | Accepted: 18/09/2008 | Published: 31/10/2008

Abstract

A thermophilic sulfate reducing bacterium was isolated from corrosion product of cooling tower of an Indian petroleum refinery and characterized as Desulfotomaculum geothermicum with morphological and biochemical characters. Gene coding dissimilatory sulfite reductase (dsrAB), a key enzyme in the sulfate reduction was sequenced and cloned in an expression vector and over expressed with rDNA technology. Purified protein was identified as dissimilatory sulfite reductase having the molecular size of 97.6 kDa with MALDI – MS and discussed.

Keywords

Sulfate reducing bacteria, biocorrosion, dissimilatory sulfite reductase

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