A gene encoding cellulase enzyme involved in carboxymethylcellulose (CMC) degradation was isolated and sequenced from the ûlamentous fungus, Trichoderma reesei. A DNA fragment of 764bp conferring cellulase activity was cloned in E. coli BL21 and it contained a 726 bp ORF which encoded a protein belonging to glycosyl hydrolase family 45 and was classified as endoglucanase. It showed a maximum cellulase activity of 11.75 IU/g, at pH 5.5 and at 37oC. The SDS–PAGE and zymogram analyses showed a protein band with a molecular weight of about 24,420 Da confirming endoglucanase expression.
Cellulase, Endoglucanase gene, cDNA, E.coli BL21 and Trichoderma reesei
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