ISSN: 0973-7510

E-ISSN: 2581-690X

Ramchander Merugu1,2 , M.P. Pratap Rudra2, Atthapu Thirupathaiah3, S. Girisham4 and S.M. Reddy4
1Department of Biochemisty, Kakatiya University, Warangal, India.
2Department of Biochemisty, Osmania University, Hyderabad, India.
3Department of Pharmaceutical Chemistry, Mahatma Gandhi University, Nalgonda, India.
4Department of Microbiology, Kakatiya University, Warangal – 506 009, India.
J Pure Appl Microbiol. 2011;5(2):1027-1029
© The Author(s). 2011
Received: 10/07/2010 | Accepted: 29/08/2010| Published: 31/10/2011
Abstract

An  extensive  and  intensive  survey  of  sewage, industrial effluents, rice  fields, freshwater and earthworm  casts  for the presence of  purple non-sulphur  bacteria  was undertaken from Warangal district of Andhra Pradesh state in India. In  all the  nine  bacterial species,  which  included  Rhodopseudomonas  palustris, R.rutila, R.acidophila, Rhodopila globiformis, Rhodospirillum  rubrum, Rsp.photometricum,  Rhodobacter sphaeroides, Rb.capsulatus, Rhodobacter sp and  Rhodocyclus gelatinosus were isolated. Among these Rhodobacter capsulatus, isolated from leather industry effluents could reduce maximum amount of chromium and was selected for further studies. Effect of alginate immobilization on reduction of chromium by Rhodobacter capsulatus was also investigated. Rb.capsulatus could reduce about 28 µM of chromium.  Chromate reduction by free cells was less and was only 19 µM. Maximum reduction of chromium by free or immobilized cells of Rb. capsulatus took place on 12th day itself. Optimal activity of reductase was shown to take place at pH 7.6 and a temperature of 38°C. Enzyme activity was found to be in soluble fraction. Significance of the above results in light of existing literature is discussed in this communication.

Keywords

Rhodobacter capsulatus, Chromate reduction, Anaerobic light, Chromate reductase

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