ISSN: 0973-7510

E-ISSN: 2581-690X

Nur Ceyhan1 , Dilek Keskin2 and Zuhal Zorlu1
1Department of Biology, Faculty of Sciences, Mugla University, Mugla, Turkey.
2Cine Vocational High School, Adnan Menderes University, Aydin, Turkey.
J Pure Appl Microbiol. 2012;6(3):1447-1453
© The Author(s). 2012
Received: 22/02/2012 | Accepted: 29/03/2012 | Published: 30/09/2012

The extracts of methanol–water, hexane and chloroform eucalyptus leaves (Eucalyptus camaldulensis Dehnh.) were screened in vitro for their antimicrobial activity against some pathogenic gram negative, gram positive bacteria and one yeast. Disk diffusion method was used for antimicrobial activity of extracts and the microwell dilution assay was used for the determination of minimal inhibitory concentration (MIC) of each extract. In addition, the composition of chloroform extratc was determined by means of GC/MS analysis. The methanol/water, hexane and chloroform exracts of E.camaldulensis leaf showed various antimicrobial activity 7-9mm, 8-11mm and 11-27 mm inhibition zone, respectively. The chloroform extracts was found to be the most effective antimnicrobial agent as compared to the other extracts. While the most sensitive bacteria was Bacillus cereus CCM 99 (27mm inhibition zone), the most resistant bacteria was Esherichia coli ATCC 35218. The methanol/water, hexane and chloroform extracts of E. camaldulensis leaf showed 1.25-5.0<µg/ml, 0.625-5.0<µg/ml and 0.039-0.625µg/ml MIC value, respectively. While the chloroform extract displayed the best activity (MIC 0.039 µg/ml) against B.subtilis ATCC 6633 and B.cereus CCM 99 and C.albicans ATCC 10239, it showed the least activity ( MIC 0.625 µg/ml) against E.coli ATCC 35218. The GC/MS analyses allowed 11 compounds to be determined; the main constituents of the Eucalyptus leaves extract were chloroform (68.5%), hexane(10.85%), ethylacetate (9.12%), ethanol (8.25%), g terpiene (0.78%), bromochloromethane  (0.59%), 1,8 cineole  (0.52 %). The data of this work indicate that extracts of eucalyptus leave from West Anatolia, especially the chloroform extracts, could be formed the basis of many applications including raw and processed food preservation, pharmaceuticals, alternative medicine, and natural therapies.


Eucalyptus camaldulensis Dehnh., leaf extract, Antimicrobial activity, Chemical composition, GC-MS analysis

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