Staphylococcus aureus is major pathogen causing mastitis in dairy milch animals including goats. The present study was carried out on 27 S. aureus isolates of milk origin from goats with clinical mastitis which included characterization of isolates for two important virulence associated genes namely clfA, responsible for clumping factor and cap, responsible for capsule. All the isolates were genotypically identified by 23S rRNA ribotyping in which a species-specific amplicon of 1250 bp was obtained. Of the 27 isolates 17 (68.38%) showed single amplicon of approximately 350 bp representing presence of cap5Kgene responsible for capsular type 5 whereas nine (34.61%) of the isolates produced amplicons of approximately 200bp representing presence of cap8K genes responsible for production of capsular type 8, whereas one isolate was non-typable for any of the two genes.In the present investigation each of the 25 isolates produced single amplicon of 1050 bp indicating no polymorphism in clfA genewhile two isolates did not produce any PCR amplicon. Epidemiological investigation of these genes is significant in terms of vaccine design and control of mastitis from the study area.
Staphylococcus aureus, cap, clfA, goat, mastitis
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