ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Madhu Yadav1 , R. Sujatha2 and Anil Kumar3
1Department of Microbiology, GSVM Medical College, Kanpur, Uttar Pradesh, India.
2Department of Microbiology, Rama Medical College, Kanpur, Uttar Pradesh, India.
3Department of Biotechnology, Rama Medical College, Kanpur, Uttar Pradesh, India.
J. Pure Appl. Microbiol., 2020, 14 (1): 375-381| Article Number: 5911
https://doi.org/10.22207/JPAM.14.1.38 | © The Author(s). 2020
Received: 18/10/2019 | Accepted: 10/02/2020 | Published: 17/02/2020
Abstract

In developing countries diarrheal illness are causing severe health problem in children up to five years of age group and it also results in causing the morbidity and mortality. Worldwide among all the bacterial pathogens diarrheagenic Escherichia coli is responsible for epidemic and endemic diarrhoea. E. coli rarely cause disease in healthy individuals and most of E. coli strains live harmlessly in the intestines. The objective of the study was to detect six categories of DEC in one set of Multiplex PCR reactions. Total number of 155 in and outdoor children stool sample with complain of diarrhoea were collected in Rama Medical College, Kanpur, India. Target genes used for amplification by Multiplex PCR were eaeA and bfpA for EPEC, elt and stla for ETEC, CVD432 for EAEC and hlyA for EHEC. Out of 155 isolates examined, 71 were E.coli isolates, out of 71 isolates 28 were ETEC, 02 were EHEC. elt gene of ETEC and hlyA gene of EHEC diarrheagenic E.coli were detected. Antibiotic sensitivity tests for diarrheagenic strains were done by Kirby Bauer Method. Antibiotics used in the study were Ampicillin, Cotrimoxazole, Ciprofloxacin, Norflox, Nalidixic acid, Cefotaxime, Chloramphenicol, Imipenem, Azithromycin, Gentamicin. In most cases ETEC and EHEC diarrheagenic isolates were found resistant to Ampicillin, Gentamicin, Cotrimoxazole and Azithromycin. Multiplex PCR, a rapid method for detecting virulent genes of DEC at a time and it also provide a platform in understanding the diarrheal diseases in a more improved manner.

Keywords

E. coli, Morbidity, Mortality, Antibiotic sensitivity, ETEC, EHEC, Endemic.

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