An alkaliphilic actinomycete strain, designated WS65, was isolated from a desert soil sample collected from Beni-Suef Governorate in Egypt, and was then subjected to polyphasic taxonomy. The strain produced substrate and aerial mycelia on different media with optimum pH for growth 9.5- 10 and no growth was recorded at pH 7. Strain WS65T contained meso-diaminopimelic acid, no diagnostic sugars, type PIII phospholipids, and MK-10 (H8) and MK-10 (H6) as the predominant menaquinones. The morphological and chemotaxonomic characteristics of the isolate were in agreement with those described for members of the genus Nocardiopsis. This was confirmed by 16S rRNA sequence comparison and phylogenetic analysis. The strain was distinguished from the closely related Nocardiopsis species by a number of phenotypic characters, but the high DNA-DNA pairing similarities didn’t support the proposal of strain WS65 (=DSM 44686T =CCTCC AA001033T) as a novel species in the genus Nocardiopsis. The 16S rRNA sequence of the strain was deposited in GenBank under the accession number AY331687.
Characterization, Nocardiopsis, alkaliphilic, desert, polyphasic taxonomy
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