ISSN: 0973-7510

E-ISSN: 2581-690X

P. Jayalakshmi, P. Suvarnalatha Devi, G. Revathi, N.D. Prasanna and S.K. Shaheen
Department of Applied Microbiology, SPMVV, Tirupati – 517 502, India.
J Pure Appl Microbiol. 2010;4(2):863-866
© The Author(s). 2010
Received: 03/04/2010 | Accepted: 24/05/2010| Published: 31/10/2010
Abstract

The Nitrogenase Fe- protein was isolated from Rhizobium sps. which was  purified and the properties similar to those of the Fe- Protein of other nitrogen fixing organisms with respect to its molecular weight, non-protein groups like Phosphates, Ribose and an adenine like unit are covalently bound to the protein. The nitrogense Fe protein is a a2 dimer containing Fe4S4 cluster. The Fe- protein was eluted with 400 mM- Nacl on to a DEAE-Cellulose column as a dark brown band at the top of the column. The molecular weight of the protein is about 61500Da.

Keywords

Nitrogenase Fe- protein, Rhizobium sps, DEAE-cellulose column, NifH gene

Article Metrics

Article View: 589

Share This Article

© The Author(s) 2010. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.