ISSN: 0973-7510

E-ISSN: 2581-690X

Mohammad Shahid , Sonika Pandey, Mukesh Srivastava, Vipul Kumar,Anuradha Singh, Shubha Trivedi, Y.K. Srivastava and Shivram
Biocontrol Laboratory, Department of Plant Pathology, CSA University of Agriculture & Technology, Kanpur-208002, Uttar Pradesh, India.
J. Pure Appl. Microbiol., 2016, 10 (1): 769-786
© The Author(s). 2016
Received: 14/11/2015 | Accepted: 13/01/2016 | Published: 31/03/2016
Abstract

Ten species of Trichoderma were isolated from the rhizospheric soil, collected from the different locations of U.P. Both morphological and molecular characterization of the isolated species was done. All the ten isolated species were screened for chitinase enzyme production on solid agar medium using bromocresol purple for developing the clear zone around colonies, and characterized due to its antagonistic effect against mycelia growth of pathogenic fungi.  The nucleotide sequences (submitted and retrieved from NCBI) of all ten Trichoderma species are analyzed through TrichOKEY 2 program for their validation post molecular identification. This has confirmed the selected sequences as specific strains of Trichoderma species.

Keywords

Trichoderma, TrichOKEY, Chitinase.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.